By Mark Danielsen (auth.), Benjamin A. Lieberman (eds.)
Research into steroid receptor biology holds nice promise for the therapy of melanoma and endocrine problems and for elucidating the basic organic mechanisms of gene expression. In Steroid Receptor tools: Protocols and Assays, a individual group of imperative investigators and their affiliates describe in step by step element a cross-section of the most recent learn recommendations to be had for learning the endocrine method. As a foundation for stylish biochemical research of receptor homes, the participants offer tools for the creation and purification of quite a few receptors, together with progesterone, glucocorticoid, and androgen. different protocols enable the reader to scan with DNA-binding features and hormone-binding assays and with using combinatorial chemistry for drug discovery. a sequence of novel equipment using the newest advances in immunochemistry, yeast two-hybrid screening, and fluorescence are integrated for the detection and research of numerous mobile proteins that impression steroid receptor effectiveness. eventually, a dialogue by means of instance offers the most up-tp-date bioinformatics instruments and analytical techniques.
well timed and hugely obtainable, Steroid Receptor equipment: Protocols and Assays presents biochemists and molecular biologists learning endocrine platforms with an entire variety of the strong instruments wanted for elucidating protein interactions with receptors and DNA, in addition to for drug discovery.
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25. , and Kahn, D. (2000) ProDom and ProDom-CG: tools for protein domain analysis and whole genome comparisons. Nucleic Acids Res. 28, 267–269. 26. , and Kahn, D. (1999) Whole genome protein domain analysis using a new method for domain clustering. Comput. Chem. 23, 333–340. 27. , Lazar, M. , and Rastinejad, F. (1998) Structural elements of an orphan nuclear receptor-DNA complex. Mol. Cell 1, 849–861. 28. Gibrat, J. , and Bryant, S. H. (1996) Surprising similarities in structure comparison. Curr.
For all-around versatility with molecular sequence data, Phylogenetic Analysis Using Parsimony (PAUP*) (19), a package developed by Swofford, is difficult to beat, especially if one has had a MacIntosh computer. Recently, PC-compatible and UNIX versions have joined the VAX/VMS and Mac OS packages. com) and menu-driven, it is the most popular phylogenetic analysis package for molecular data. It is the package that my lab uses almost exclusively for phylogenetic analyses. PAUP* has a large number of programs besides those that are parsimony-based and will read a wide range of data input files, including Nexus, PHYLIP, and FASTA.
Htm. Bioinformatics of Nuclear Receptors 21 Acknowledgments The author wishes to thank J. Jonklaas and E. Martinez for comments on the manuscript. Work in the laboratory of M. D. is supported by grants from the American Heart Association, the National Kidney Foundation, and the Susan G. Komen Breast Cancer Foundation National Race for the Cure. References 1. , Moore, D. , Vanden Heuvel, J. , et al. (1998) The Nuclear Receptor Resource: a growing family. Nucleic Acids Res. 26, 239–241. 2. Wheeler, D.