Methods of Biochemical Analysis by D. Glick (ed.)

By D. Glick (ed.)

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In 1953, Boyland, Manson, Solomon, and Wiltshire (10) isolated this acid from the urine of normal rabbits as well as from that of rabbits injected with naphthalene. One of the most useful techniques for the investigation of phenolic constituents of biological material is paper chromatography (see Section II,3) which may be used for identification or estimation. A preliminary investigation (21,26) of normal rabbit urine showed the presence of m- and phydroxybenzoic acids, p-hydroxyphenylacetic acid, catechol, 2,3-, 3,4-, and 2,5-dihydroxybenzoic acids, vanillic acid, and p-cumaric acid as well as several unidentified substances reacting like phenolic compounds.

2. Ethereal Sulfate.. . . . . . . . . . . . 3. Glucuronic Acid.. . . . References. . . . . . . . . . . . . . . . . . . . . . . . . . 27 28 31 31 32 32 37 40 41 41 42 50 I. INTRODUCTION The total number of phenolic compounds which have been identified as constituents or metabolites of living organisms is very large. In an article of this scope a comprehensive survey of these compounds would scarcely be possible. Such a survey, indeed, would be of only partial interest to the average reader whose studies tend to be confined either to the animal or to the plant kingdom.

HELLERMAN 3. General Remarks It is evident that caution should be exercised in interpreting the results of application of the nitroprusside reaction. As already pointed out, the reaction is not specific for - S H groups. In studies of proteins consideration should be given to the composition of the solution in which the nitroprusside test is applied. The high pH could have an effect on the protein, such as denaturation; the high ionic strength obtaining in some procedures might have a similar effect.

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