By Irma H. Russo M.D. (auth.), Jose Russo (eds.)
There isn't any doubt that the appearance of immunocytochemical ideas, through improving our skill to become aware of particular mobilephone items or markers, has opened new avenues within the realizing of human illnesses, and in our skill to accomplish larger prognosis in surgical pathology. The speedy improvement of this box has ended in hundreds of thousands of courses within the literature relating to immunocytochemistry in diagnostic pathology. This explosion of data makes precious guides summarizing what are the most markers on hand and the way they are often utilized in the analysis of tumors. the necessity of a extra equipped and based wisdom was once obvious in the course of the workshop in Immunocytochemistry of Tumor analysis that happened within the urban of Detroit an October 1984. This publication is the results of that workshop within which 22 chapters are focusing an the most topic of differential prognosis of tumors. Jose Russo, M.D. Editor xiii ACKNOWLEDGEMENTS I desire to thank my many affiliates on the Michigan melanoma starting place for his or her assist in the coaching of this manuscript. I provide because of Dr.
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Extra info for Immunocytochemistry in Tumor Diagnosis: Proceedings of the Workshop on Immunocytochemistry in Tumor Diagnosis Detroit, Michigan—October 3–5, 1984
That this may be a more sensitive means of identifying adenocarcinoma cells in effusive fluids has been suggested by the results of others who used similar antibodies (66,67). 45 FIGURE 15. Poorly differentiated adenocarcinoma of ovarian or1g1n, metastatic to omentum.
J Clin Pathol 33:356-360, 1980. van Muijen GNP, Ruiter DJ, Ponec M, et al: Monoclonal antibodies with different specificities against cytokeratins. Am J Pathol 114:9-17, 1984. Debus E, Moll R, Franke WW, et al: Immunohistochemical distinction of human carcinomas by cytokeratin typing with monoclonal antibodies. Am J Pathol 114:121-130, 1984. Hautzer NW, Wittkuhn JF, McCaughey WRE: Trypsin digestion in immunoperoxidase staining. J Histochem Cytochem 28:52-53, 1980. Imam A, Taylor CR, Tokes ZA: Immunohistochemical study of the expression of human milk-fat-globule membrane glycoprotein-70.
For example, in a female with a tumor of unknown origin, it would be logical to stain with antibody against secretory epithelial mernbrane antigen and Iactalbumin (or other breast related antigens), which, following absorption, may be made relatively specific for breast tissue (21-24); whereas staining with antibody against prostate-specific acid phosphatase would make little sense. In the male, of course, the situation is reversed; the probability of breast cancer is very small and the primary choice would be antibody against prostate-specific acid phosphatase (PSAP) or prostate-specific epithelial antigen (PSA) (25-27).