Histochemical Techniques by J. D. Bancroft (Auth.)

By J. D. Bancroft (Auth.)

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It was capable of reaching —30°C and an accurate thermostat allowed control of temperature between - 5 ° C and - 3 0 ° C . 1 The Slee motorized cryostat containing the Slee retracting microtome. Reproduced by courtesy of Slee Medical Equipment Ltd. e. coarse advance, thickness setting, operating handle and anti-roll 32 DEVELOPMENT OF CRYOSTATS plate) were all external and could be operated through the sides or top of the chamber. T h e anti-roll plate was attached to the microtome and not, as in the Coons cryostat, to the knife.

T h e important difference in freeze dried material, apart from the embedding in paraffin wax, is that the disruptive effects of thawing of the frozen sections is avoided, all the unbound water having been removed while the tissue is below freezing point. T h e importance of this fact is frequently overlooked in the appraisal of these two techniques. R e a s o n s for f r e e z e d r y i n g T h e standard methods of tissue preparation involving fixation, dehydration, clearing and embedding in paraffin wax have a n u m b e r of drawbacks for the histochemist.

T h e controls are mounted on the front panel of the cabinet with two gauge dials giving the module temperature and the vapour pressure. T h e controls allow the operator to isolate the drying chamber from the vacuum p u m p and to regulate the temperature of the thermomodules from + 6 0 ° C to — 60°C. T h e vapour trap usually used in the freeze dryer is phosphorus pentoxide, contained in two boats placed near the vacuum line.

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