By Marco F. Schmidt
This quantity presents a concise and technical dialogue of lately built techniques to beat demanding situations, corresponding to pharmacodynamics and pharmacokinetics problems, in miRNA drug discovery. those recommendations disguise anti-sense brokers concentrating on miRNA which are utilized in complex formulations or are chemically optimized to extend supply; small molecule miRNA modulators to beat anti-sense brokers’ obstacles; common enhancers of miRNA maturation; and Argonaute 2 protein and its pharmacokinetic parameters. Written within the hugely winning Methods in Molecular Biology series layout, chapters contain introductions to their respective themes, lists of the mandatory fabrics and reagents, step by step, effortlessly reproducible laboratory protocols, and pointers on troubleshooting and heading off recognized pitfalls.
Cutting-edge and thorough, Drug objective miRNA: equipment and Protocols is a necessary source for somebody drawn to the ever-evolving box of miRNA drug discovery.
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Extra info for Drug Target miRNA: Methods and Protocols
Science 336:1037–1040 48. Elkayam E et al (2012) The structure of human Argonaute-2 in complex with miR20a. Cell 150:100–110 49. Tan GS et al (2012) Small molecule inhibition of RISC loading. ACS Chem Biol 7:403–410 50. Masciarelli S et al (2014) A small-molecule targeting the microRNA binding domain of Argonaute 2 improves the retinoic acid differentiation response of the acute promyelocytic 22 51. 52. 53. 54. 55. Marco F. Schmidt leukemia cell line NB4. ACS Chem Biol 9:1674–1679 Wang Y et al (2009) Nucleation, propagation, and cleavage of target RNAs in Ago silencing complexes.
Tan GS et al (2012) Small molecule inhibition of RISC loading. ACS Chem Biol 7:403–410 50. Masciarelli S et al (2014) A small-molecule targeting the microRNA binding domain of Argonaute 2 improves the retinoic acid differentiation response of the acute promyelocytic 22 51. 52. 53. 54. 55. Marco F. Schmidt leukemia cell line NB4. ACS Chem Biol 9:1674–1679 Wang Y et al (2009) Nucleation, propagation, and cleavage of target RNAs in Ago silencing complexes. Nature 461:754–761 Kole R, Krainer AR, Altman S (2012) RNA therapeutics: beyond RNA interference and antisense oligonucleotides.
The column on the right offers advanced filtering options. All results can be filtered based on numerous features, including species, method type, method, regulation type, validation type, and publication year (see Note 7). For instance, in order to select all the entries that have been also validated by reporter gene assays, press “Method” on the filter pane and check the “Luciferase Reporter Assay” check box. All entries that have been validated with this technique are shown in the results pane.