Cumitech 44: Nucleic Acid Amplification Tests for Detection by Susan E. Sharp

By Susan E. Sharp

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Extra resources for Cumitech 44: Nucleic Acid Amplification Tests for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae

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Ibecheole, and A. Campana. 2001. Combined screening for Chlamydia trachomatis and squamous intra-epithelial lesions using a single liquidbased cervical sample. Hum. Reprod. 16:2206–2210. 7. , and M. Jones. 2002. Comparison of M4 and M4RT media for transporting cervical swab samples for PCR detection of Chlamydia trachomatis and Neisseria gonorrhoeae. Ann. Clin. Lab. Sci. 32: 61–64. 39 15. Black, C. M. 1997. Current methods of laboratory diagnosis of Chlamydia trachomatis infections. Clin. Microbiol.

Gonorrhoeae or C. trachomatis result, and the requirement to report results to state health departments and partners of infected individuals, care needs to be taken to ensure the accuracy of results at all times. A study of five laboratories performing C. trachomatis NAATs on urine samples from asymptomatic men has shown variance within and between laboratories in the performance of similar assays. The authors recommend that strict adherence to quality control, proper training, and continuous monitoring of test performance characteristics are essential (112).

3b Culture; discrepants, with DFA and PCR PACE 2; discrepants with PCR Culture; discrepants with DFA Culture or PCR Specificity (%) Positive predictive value (%) CUMITECH 44 Sensitivity (%) Comparison method Prevalence (%) Specimen types No. of subjects, sex, sample site Reference Table 11. Recent studies evaluating Digene Hybrid Capture 2 assays for detection of N. 8 Leber et al. Negative predictive value (%) 28 Future Directions It would be most efficient to be able to perform multiple tests from a single specimen in addition to C.

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