Cattle Embryo Transfer Procedure: An Instructional Manual by John L. Curtis

By John L. Curtis

This complete, step by step laboratory education handbook brings all of the parts for a winning embryo move software jointly in an easy, equipped, illustrated structure. For the final a number of a long time, man made insemination has allowed genetic growth to be completed fairly speedy throughout the frequent and effective use of frozen semen. a result of development of embryo move (ET) recommendations, cows can produce many offspring. A extra fast genetic achieve is accomplished which enhances a synthetic insemination software.

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Extra info for Cattle Embryo Transfer Procedure: An Instructional Manual for the Rancher, Dairyman, Artificial Insemination Technician, Animal Scientist, and Veter

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7. Wipe the vulva clean with a paper towel. 8. Part the vulva by placing a clean, folded paper towel between the vulva lips. 9. Pass the breeding rod through the cervix, and stop when the rod has just entered the uterus. 10. Slowly (five seconds) plunge semen into the uterus. Avoid pushing the rod forward while plunging! 11. Withdraw the insemination rod. Record the breeding by noting the complete name and registration number of both sire a i d dam, sire code number, date, and name of inseminator.

In order to properly monitor horn filling and emptying, it is crucial that the uterine horn is manipulated so that a majority of the horn lies in the palm of your hand. This permits sensitive finger-tip palpation of the small diameter anterior end of the horn (Figure 38), where embryos are usually located 7 days after estrus. Once you arc comfortable with the position of the horn, open clamp #2 which allows PBS to enter the uterus. Horn fill/ expansion is constantly monitored by gently touching and tapping the entire length of the horn while it fills.

11. Withdraw the insemination rod. Record the breeding by noting the complete name and registration number of both sire a i d dam, sire code number, date, and name of inseminator. 1) COLLECTION DATE AND RECIPIENT IDENTIFICATION Bovine embryo collection is generally attempted on day 7 after estrus, which yield,s a development stage suitable for freezing. Embryos are frequently located in the oviducts prior to day 6 and therefore cannot be collected by the nonsurgical recovery technique. 5, the clear sphere (zona pellucida, Figures 12 and 13) surrounding the embryonic cellular mass has ruptured, leaving an embryo too mature for freezing.

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